Diversity of Culturable Bacteria Isolated From the Feces of Wild <i>Anopheles darlingi</i> (Diptera: Culicidae) Mosquitoes From the Brazilian Amazon

Abstract Microorganisms living in the midgut of Anopheles mosquitoes have been studied to fight vector-borne diseases, such as malaria. Studies on microbiota Neotropical darlingi, most important Brazilian vector for malaria, reported same purpose. Our aims were isolate and identify culturable bacteria from An. darlingi mosquito guts through their feces estimate species richness frequency distribution sampled bacteria. Sixty wild females captured at two rural locations, near Porto Velho, Rondônia, Brazil. Bacteria isolated feces, which collected using cages permit collection LB nutrient agar plates. bacterial colonies stored glycerol −80°C. identified by sequencing 16S rRNA gene obtained PCR Sanger sequencing. To aid identification, MALDI-TOF, VITEK2, BBL Crystal used complementary protocols. The sequences 60 isolates compared deposited GenBank (NCBI) BLAST. Homology greater than 97% between query subject was criteria assigning identity each isolate. Fourteen eight different genera among isolates. frequent Serratia liquefaciens (20%) marcescens (15%). Due established apathogenicity according previous studies, we suggest Pantoea suitable paratransgenesis development malaria Amazon.