Development of a Brassica seed cDNA microarray

cDNA Microarray Data Normalization

A Proteomic Analysis of Seed Development in Brassica campestri L

To gain insights into the protein dynamics during seed development, a proteomic study on the developing Brassica campestri L. seeds with embryos in different embryogenesis stages was carried out. The seed proteins at 10, 16, 20, 25 and 35 DAP (days after pollination), respectively, were separated using two-dimensional gel electrophoresis and identities of 209 spots with altered abundance were d...

Analysis of Gene Expression during Brassica Seed Germination Using a Cross- Species Microarray Platform

We have developed an approach to facilitate the use of model organism microarrays in related, nonmodel organisms. We demonstrate the method by using Arabidopsis oligonucleotide microarrays to analyze gene expression in Brassica. Probes with low hybridization signals to Brassica genomic DNA were excluded from the transcriptional analysis of an Arabidopsis microarray at the software level, formin...

Ethylene Production during Development of Mustard (Brassica juncea) and Canola (Brassica napus) Seed.

An open, continuous flow system was used to investigate ethylene production during degreening of maturing seed of mustard (Brassica juncea cv Cutlass and cv Lethbridge 22A) and canola (Brassica napus cv Westar and cv Alto). Isolated mustard seed evolved higher amounts of ethylene than those of canola, and this was particularly evident both early in embryogeny and later during the desiccation ph...

Construction of the seed-coat cDNA microarray and screening of differentially expressed genes in barley.

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, ...