Delta-type DNA polymerase characterized fromDrosophila melanogasterembryos
نویسندگان
چکیده
منابع مشابه
DNA repair synthesis in human fibroblasts requires DNA polymerase delta.
When UV-irradiated cultured diploid human fibroblasts were permeabilized with Brij-58 then separated from soluble material by centrifugation, conservative DNA repair synthesis could be restored by a soluble factor obtained from the supernatant of similarly treated HeLa cells. Extensive purification of this factor yielded a 10.2 S, 220,000-dalton polypeptide with the DNA polymerase and 3'- to 5'...
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The fidelity of Schizosaccharomyces pombe DNA polymerase delta was measured in the presence or absence of its processivity subunits, proliferating cell nuclear antigen (PCNA) sliding clamp and replication factor C (RFC) clamp-loading complex, using a synthetic 30-mer primer/100-mer template. Synthesis by pol delta alone was distributive. Processive synthesis occurred in the presence of PCNA, RF...
متن کاملDNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.
DNA polymerase delta (pol delta) plays an essential role in DNA replication, repair, and recombination. We have purified pol delta from Schizosaccharomyces pombe more than 10(3)-fold and demonstrated that the polymerase activity of purified S. pombe pol delta is completely dependent on proliferating cell nuclear antigen and replication factor C. SDS/PAGE analysis of the purified fraction indica...
متن کاملDevelopment of Selective Inhibitors of DNA Polymerase Delta: A Thesis
This thesis is divided into three parts , united by the theme of the development of selective inhibitors of mammalian cell DNA polymerase delta (pol S). The first part consists of an investigation of the cytotoxic mechanism(s) of certain 2-substituted adenine analogs , selected on the basis of their inhibitory properties towards DNA polymerase alpha (pol a) and mammalian cell DNA synthesis. The...
متن کاملHuman DNA polymerase delta double-mutant D316A;E318A interferes with DNA mismatch repair in vitro
DNA mismatch repair (MMR) is a highly-conserved DNA repair mechanism, whose primary role is to remove DNA replication errors preventing them from manifesting as mutations, thereby increasing the overall genome stability. Defects in MMR are associated with increased cancer risk in humans and other organisms. Here, we characterize the interaction between MMR and a proofreading-deficient allele of...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.21.5779