Curcumin-enriched Gemini surfactant nanoparticles exhibited tumoricidal effects on human 3D spheroid HT-29 cells in vitro

Abstract Background Here, we examined the tumoricidal effect of Gemini surfactant nanoparticles enriched with curcumin on 3D spheroid HT-29 cells. The delivery and other phytocompounds to tumor niche is an important challenge. Methods Spheroid cells were generated by using a conventional hanging drop method exposed different concentrations Gemini-curcumin nanoparticles. changes in integrity cell viability evaluated measuring diameter LDH release, respectively. uptake was detected flow cytometry assay. Flow cytometric Rhodamine 123 efflux also performed. Migration capacity analyzed Transwell insert By real-time PCR analysis Western blotting, studied expression level MMP-2, -9, Vimentin, E-cadherin genes. Results had potential disintegrate spheroids decrease central density compared control group ( p < 0.05). These coincided enhanced release increase nanoparticle concentration Data highlighted ability synthetic dose-dependent manner. We found reduced treated Nanoparticles significantly decreased metastasis epithelial-mesenchymal transition (EMT) rate suppression MMP-2 MMP-9, induction Conclusion Our data confirmed that has suppress proliferation inhibit vitro.