CONTROLLED PRODUCTION OF PROLIFERATING SOMATIC CELL HYBRIDS
نویسندگان
چکیده
منابع مشابه
Controlled Production of Proliferating Somatic Cell Hybrids
The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolaye...
متن کاملSomatic-cell hybrids producing antibodies against CEA.
Monoclonal antibodies to carcinoembryonic antigen (CEA) promise improved specificity for the measurement of this widely expressed human cancer antigen. A mouse monoclonal antibody binds weakly to CEA in perchloric acid extracts of tumour but strongly to CEA similarly isolated from serum, and its spectrum of cancer detection differs from conventional antisera.
متن کاملModification of myc gene amplification in human somatic cell hybrids.
In order to study whether cell fusion would modify the DNA copy number of an amplified oncogene, somatic cell hybrids were made between the human neuroepithelioma cell line MCIXC and HeLaCOT human adenocarcinoma cells. MCIXC contains approximately 21 copies of the c-myc oncogene and HeLaCOT contains approximately 5 copies relative to the control. All hybrid clones investigated displayed a marke...
متن کاملGrowth characteristics of Burkitt somatic cell hybrids in vitro.
SUMMARY Burkitt somatic cell hybrids (D98/HR-l) synthesize both the A and B forms of glucose 6-phosphate dehydrogenase, which were originally derived from the two fused human cells. The A form is from D98 cells and the B form was supplied by the P3J-HR-l cells. The formation of a heteropolymer of glucose 6-phosphate dehydrogenase in the hybrid proves that a hybrid was obtained. Selection of hyb...
متن کاملSuppression of the transformed phenotype in somatic cell hybrids.
Somatic cell hybrids between mouse mammary tumour cells (TA3B) and diploid rat embryo fibroblasts (REF) or between TA3B and Syrian hamster sarcoma cells (BI) were examined for the in vitro characteristics of transformed cells as soon as possible after cell fusion. Unlike the parental tumour cells as three of four TA3B X REF and five BI X TA3B independent hybrid lines had low colony-forming effi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1970
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.45.1.74