Complementation of a trpE deletion in Escherichia coli by Spirochaeta aurantia DNA encoding anthranilate synthetase component I activity
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چکیده
منابع مشابه
Hydroxamate formation by anthranilate synthetase of Escherichia coli K12.
HO' H /H2 0 \ I dOOH chorismic acid anthranilic acid (Gibson and Gibson, 1964; Somerville, unpublished results). In Escherichia coli the catalytically active species is a protein complex formed by aggregation of the products of the E and D genes of the tryptophan operon (Ito and Yanofsky, 1966). Anthranilate synthetase is one of a class of amidotransferase enzymes where L-glutamine serves as th...
متن کاملAnthranilate synthetase, an enzyme specified by the tryptophan operon of Escherichia coli: purification and characterization of component I.
A procedure employed in the purification of anthranilate synthetase component I of Escherichia coli is described. The purified component appears homogeneous by starch gel electrophoresis and by sedimentation analysis. A molecular weight of 60,000 was estimated by gel filtration of Sephadex G-100. This value is consistent with the molecular weight estimated from the sedimentation and diffusion c...
متن کاملSpirochaeta aurantia has diacetyl chloramphenicol esterase activity.
The free-living spirochete Spirochaeta aurantia was nearly as susceptible to diacetyl chloramphenicol, the product of chloramphenicol acetyltransferase, as it was to chloramphenicol itself. This unexpected susceptibility to diacetyl chloramphenicol was wholly or partly the consequence of intrinsic carboxylesterase activity, as indicated by high-performance liquid chromatography, thin-layer chro...
متن کاملAnthranilate Synthetase From Escherichia coli SJHI: Purification and Some Properties
Abstract: A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially us...
متن کاملChemotaxis mutants of Spirochaeta aurantia.
Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior similar to that of the wild-type strain. The che-101 and che-400 mutants were essent...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1987
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.169.8.3764-3769.1987