Comparison of Lysis Buffers for Saliva DNA Extraction using Modified Magnetic-Silica Nanoparticle Beads

Our previous study revealed that modified MAGSi (magnetic-silica nanopartivel beads) had various sizes and shapes affected the DNA extraction process. In this study, we combined our with several kinds of lysis buffer for to analyze result. Human saliva was extracted using three MAGSi. Each them made differently. We used ATL buffer, Triton Buffer, without cell lysis. The concentration purity were analyzed spectrophotometry. Extracted also as template amplification beta globin gene. This MAGSi-1 provided best result purity. produced less yield value but it more consistent. different All can be human Further studies are still needed determine effectiveness consistency