Colorimetric ultramicro method for the determination of free fatty acids

Colorimetric Ultramicro Method for the Determination of Free Fatty Acids.

Semiautomated Method for the Colorimetric Determination of Plasma Free Fatty Acids.

A semiautomated procedure is described for the estimation of plasma free fatty acids. The method requires the preliminary preparation of a phospholipid-free plasma lipid extract which is then analyzed automatically by a colorimetric procedure based on the solubility of copper soaps in chloroform, with subsequent complexing of the copper with diethyldithiocarbamate and measurement of the extinct...

Automated colorimetric determination of free fatty acids in biologic fluids.

HE MOST FREQUENTLY USED procedure for the determination of free fatty acids (FFA) in body fluids or biologic media is that of Dole (4). This method is based on the extraction of fatty acids with organic solvents followed by titration with dilute alkali. Despite improvements in specificity (3, 5, 6), this method is subject to error resulting from dissolved atmospheric carbon dioxide. When applie...

Colorimetric Determination of Free Fatty Acids in Biological Fluids.

Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole’s extraction mixture were obtained. Phospholipids are not extracted by the procedure descr...

Rapid colorimetric micromethod for free fatty acids.

Free fatty acids (FFA), the uranyl ion, and the basic dye Rhodamine B form colored complexes, which are extractable into toluene or benzene. Fatty acids of different chain lengths above C(10) and different degrees of unsaturation gave constant molar yield. Complexes in toluene alone are unstable, especially in the light, but a small amount of aqueous uranyl acetate stabilizes them sufficiently ...