Co-transformation of bean callus using high-velocity microprojectiles- mediated DNA transfer
نویسندگان
چکیده
منابع مشابه
Transfer of foreign genes into intact maize cells with high-velocity microprojectiles.
This report describes a process for delivering foreign genes into maize cells that does not require the removal of cell walls and is capable of delivering DNA into embryogenic and nonembryogenic tissues. Plasmid harboring a chimeric chloramphenicol acetyltransferase (CAT) gene was adsorbed to the surface of microscopic tungsten particles (microprojectiles). These microprojectiles were then acce...
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Immature soybean (Glycine max L.) embryos from commercially important cultivars were the targets of rapidly accelerated, DNA-coated, gold particles. Protoplasts were prepared from these tissues and propagated in culture under selection conditions for the introduced neomycin phosphotransferase II gene. Kanamycin-resistant calli were obtained at a rate of approximately 10(-5). Enzyme assays and S...
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ژورنال
عنوان ژورنال: International Journal of Horticultural Science
سال: 2001
ISSN: 1585-0404
DOI: 10.31421/ijhs/7/1/253