Cloning and expression of the thermostable pullulanase gene fromThermussp. strain AMD-33 inEscherichia coli
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چکیده
منابع مشابه
Purification and Characterization of Thermostable Pullulanase from Bacillus stearothermophilus and Molecular Cloning and Expression of the Gene in Bacillus subtilis.
A thermostable pullulanase (alpha-dextrin 6-glucanohydrolase [EC 3.2.1.41]) from a newly isolated Bacillus stearothermophilus strain (TRS128) was purified and characterized. The enzyme hydrolyzed (1-->6)-alpha-d-glucosidic linkages of pullulan to produce maltotriose, and the optimum temperature was 65 degrees C. About 90% of the enzyme activity was retained after treatment at 65 degrees C for 6...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1988
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1988.tb02762.x