Click chemistry with DNA

Click chemistry with DNAw

DNA extraction method with improved efficiency and specificity using DNA methyltransferase and "click" chemistry.

In an attempt to develop an alternative method to extract DNA from complex samples with much improved sensitivity and efficiency, here we report a proof-of-concept work for a new DNA extraction method using DNA methyltransferase (Mtase) and "click" chemistry. According to our preliminary data, the method has improved the current methods by (i) employing a DNA-specific enzyme, TaqI DNA Mtase, fo...

Site-specific incorporation of diamondoids on DNA using click chemistry.

A simple and robust solid phase synthetic method for the ligation of diamondoids on the phosphate backbone of DNA with "click" chemistry using [Cu(CH(3)CN)(4)]PF(6) without a stabilizing ligand is reported. It was found that as the size of dimondoid increased, a corresponding increase in melting temperature was observed.

Fluorescence imaging of chromosomal DNA using click chemistry

Chromosome visualization is essential for chromosome analysis and genetic diagnostics. Here, we developed a click chemistry approach for multicolor imaging of chromosomal DNA instead of the traditional dye method. We first demonstrated that the commercially available reagents allow for the multicolor staining of chromosomes. We then prepared two pro-fluorophore moieties that served as light-up ...

Modification of DNA-templated conductive polymer nanowires via click chemistry.

DNA templated nanowires of a pentynyl-modified poly2-(2-thienyl)-pyrrole undergo functionalisation via"click chemistry" and retain their 1D-nanostructure and conductive properties.