Chemistry of the Histochemical Phosphatase Reaction.
نویسندگان
چکیده
منابع مشابه
Histochemical Localization of Alkaline Phosphatase in The
CHEMICAL investigations have shown that alkaline phosphatase is present in the retina (De Conciliis, 1934; Reis, 1951). In these investigations the whole retina was homogenized and the enzyme estimated in extract. Obviously the estimations of enzyme activity by such a necessarily gross method give no idea of the exact distribution of the enzyme in this, functionally, so finely differentiated ti...
متن کاملThe histochemical demonstration of alkaline phosphatase in unfixed frozen sections.
Although, ideally, enzyme histochemistry should be performed on unfixed sections, so as to avoid potential artifacts inherent in chemical fixation, it is usually considered necessary to fix sections so as to stop the 'soluble' enzymes from becoming lost into the incubation medium. However, provided that suitable frozen sections are used, alkaline phosphatase can be demonstrated fully in unfixed...
متن کاملHistochemical Study of Alkaline Phosphatase Activity in the Chicken Intestine
The distribution of non-specific alkaline phosphatase (AP) in normal duodenum, rest small intestine and caecum of chicken has been studied by a catalytic histochemistry method. Embryos and chickens ranging between day 11 prior to and day 60 after hatching were used and the findings of AP reaction were correlated with the age. In the small intestine, during foetal life, the AP activity started t...
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The changes of the adrenal cortex caused by corticosteroids and ACTH, were studied histochemically using T.T.C. (2-3-5 triphenyl tetrazolium chloride), one of the tetrazolium compounds. T.T.C. is a water soluble and colorless crystalline substance, changes unsoluble in water, soluble in oil, and forms a pink colored precipitation (formazan) by a reducing process. Zweifach et al. (1951) incubate...
متن کاملFactors limiting the histochemical reaction of choline dehydrogenase activity.
~ 113-The histochemical reaction of choline dehydrogenase was completely inhibited by the pretreatment of cryostat sections with either absolute acetone or lO<yo water-in-acetone at room temperature (25°C). The temperature of the organic solvents was critical in the inhibition of the staining for choline dehydrogenase. As the temperature was lowered, the inhibitory effects of the pretreatment d...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1951
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.05-0194