Characterization of yeast iso-1-cytochrome c mRNA.
نویسندگان
چکیده
منابع مشابه
Structural Characteristics of Stable Folding Intermediates of Yeast Iso-1-Cytochrome-c
Cytochrome-c (cyt-c) is an electron transport protein, and it is present throughout the evolution. More than 280 sequences have been reported in the protein sequence database (www.uniprot.org). Though sequentially diverse, cyt-c has essentially retained its tertiary structure or fold. Thus a vast data set of varied sequences with retention of similar structure and fun...
متن کاملstructural characteristics of stable folding intermediates of yeast iso-1-cytochrome-c
cytochrome-c (cyt-c) is an electron transport protein, and it is present throughout the evolution. more than 280 sequences have been reported in the protein sequence database (www.uniprot.org). though sequentially diverse, cyt-c has essentially retained its tertiary structure or fold. thus a vast data set of varied sequences with retention of similar structure and function makes it a primary ca...
متن کاملThe mutational alteration of the primary structure of yeast iso-1-cytochrome c.
A benzidine-HzOz staining procedure and genetic tests were used for the detection of cytochrome c-deficient mutants of the cyl, cy2, cy3, cy4, and cy6 loci. The chromosomal gene, CY1, was shown to encode the primary structure of iso-l-cytochrome c from bakers’ yeast. Sixteen cyl mutants were isolated after examining over 1 million colonies. These cyl mutants could be categorized as follows: (a)...
متن کاملA mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c.
The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in the mRNA. The amount of the altered protein was diminished to 20% of the wild-t...
متن کاملDenaturation and renaturation of self-assembled yeast iso-1-cytochrome c on Au.
We have made surface plasmon resonance (SPR) measurements of yeast iso-1-cytochrome c (Cyt c) on a gold surface. Angle-resolved SPR curves are recorded as a function of urea concentration before and after self-assembly of the Cyt c. Exposure to a urea solution causes denaturation of Cyt c, which shifts the minimum in the SPR curve to a larger angle and decreases the signal amplitude. The Gibbs ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43544-2