Calcium transients associated with the T type calcium current in myotubes.
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چکیده
منابع مشابه
Calcium transients associated with the T type calcium current in myotubes
Immature skeletal muscle cells, both in vivo and in vitro, express a high density of T type calcium current and a relatively low density of the dihydropyridine receptor, the protein thought to function as the Islow calcium channel and as the voltage sensor for excitation-contraction coupling. Although the role of the voltage sensor in eliciting elevations of myoplasmic, free calcium (calcium tr...
متن کاملMeasurement of calcium transients and slow calcium current in myotubes
The purpose of this study was to characterize excitation-contraction (e-c) coupling in myotubes for comparison with e-c coupling of adult skeletal muscle. The whole cell configuration of the patch clamp technique was used in conjunction with the calcium indicator dye Fluo-3 to study the calcium transients and slow calcium currents elicited by voltage clamp pulses in cultured myotubes obtained f...
متن کاملSlow inactivation of L-type calcium current distorts the measurement of L- and T-type calcium current in Purkinje myocytes
We have examined slow inactivation of L-type calcium current in canine Purkinje myocytes with the whole cell patch clamp technique. Slow inactivation is voltage dependent. It is negligible at -50 mV but can inactivate more than half of available iCaL at -10 mV. There are two major consequences of this slow inactivation. First, standard protocols for the measurement of T-type current can dramati...
متن کاملSlow Inactivation of L-Type Calcium Current Distorts the Measurement of L- and T-Type Calcium Current in Purkinje Myocytes
We have examined slow inactivation of L-type calcium current in canine Purkinje myocytes with the whole cell patch clamp technique. Slow inactivation is voltage dependent. It is negligible at -50 mV but can inactivate more than half of available icaL at 1 0 inV. There are two major consequences of this slow inactivation. First, standard protocols for the measurement of T-type current can dramat...
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The skeletal muscle L-type calcium channel or dihydropyridine receptor (DHPR) plays an integral role in excitation-contraction (E-C) coupling. Its activation initiates three sequential events: charge movement (Q(r)), calcium release, and calcium current (I(Ca,L)). This relationship suggests that changes in Q(r) might affect release and I(Ca,L). Here we studied the effect of gabapentin (GBP) on ...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1994
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.104.6.1113