Bypassing Sir2 and O-Acetyl-ADP-Ribose in Transcriptional Silencing
نویسندگان
چکیده
منابع مشابه
Hydrolysis of O-Acetyl-ADP-ribose Isomers
O-Acetyl-ADP-ribose (OAADPr), produced by the Sir2-catalyzed NAD -dependent histone/protein deacetylase reaction, regulates diverse biological processes. Interconversion between two OAADPr isomers with acetyl attached to the C-2 and C-3 hydroxyl of ADP-ribose (ADPr) is rapid. We reported earlier that ADP-ribosylhydrolase 3 (ARH3), one of three ARH proteins sharing structural similarities, hydro...
متن کاملThe 39-kDa poly(ADP-ribose) glycohydrolase ARH3 hydrolyzes O-acetyl-ADP-ribose, a product of the Sir2 family of acetyl-histone deacetylases.
The silent information regulator 2 (Sir2) family of NAD-dependent N-acetyl-protein deacetylases participates in the regulation of gene silencing, chromatin structure, and longevity. In the Sir2-catalyzed reaction, the acetyl moiety of N-acetyl-histone is transferred to the ADP-ribose of NAD, yielding O-acetyl-ADP-ribose and nicotinamide. We hypothesized that, if O-acetyl-ADP-ribose were an impo...
متن کاملDrosophila poly(ADP-ribose) glycohydrolase mediates chromatin structure and SIR2-dependent silencing.
Protein ADP ribosylation catalyzed by cellular poly(ADP-ribose) polymerases (PARPs) and tankyrases modulates chromatin structure, telomere elongation, DNA repair, and the transcription of genes involved in stress resistance, hormone responses, and immunity. Using Drosophila genetic tools, we characterize the expression and function of poly(ADP-ribose) glycohydrolase (PARG), the primary enzyme r...
متن کاملSynthesis and biochemical evaluation of O-acetyl-ADP-ribose and N-acetyl analogs.
Synthetic routes for the preparation of O-acetyl-ADP-ribose and two novel non-hydrolyzable analogs containing an N-acetyl are described and shown to interact with the macro domain of histone protein H2A1.1.
متن کاملHydrolysis of O-acetyl-ADP-ribose isomers by ADP-ribosylhydrolase 3.
O-acetyl-ADP-ribose (OAADPr), produced by the Sir2-catalyzed NAD(+)-dependent histone/protein deacetylase reaction, regulates diverse biological processes. Interconversion between two OAADPr isomers with acetyl attached to the C-2″ and C-3″ hydroxyl of ADP-ribose (ADPr) is rapid. We reported earlier that ADP-ribosylhydrolase 3 (ARH3), one of three ARH proteins sharing structural similarities, h...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2008
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2008.06.020