Bioengineering Advanced Artificial Antigen Presenting Cells for Immunotherapy
نویسندگان
چکیده
Abstract Promising cytotoxic (CD8 +) T cell-based cancer therapies have invigorated recent efforts to develop highly tunable, robust, and cost-effective artificial antigen presenting cells (aAPCs) for cell stimulation both ex vivoand in vivo. Natural APCs, such as dendritic (DCs), can be licensed deliver optimal activation signals (peptide-MHC S1, costimulatory S2, cytokine S3) CD8 +T with CD4 help. While numerous studies demonstrate that “un-helped” mount defective responses, many existing overlook this handicap the function/persistence of resulting cells. The objective study is synthesize a tri-signal aAPC couples cell/DC interaction stimulate anti-tumor Using novel blends poly(lactic-co-glycolic acid) (PLGA) cationic poly(beta-amino-ester) (PBAE) polymers, we synthesized double emulsion microscale aAPCs containing S3 (murine IL-2) encapsulated into particle core, S1 (anti-CD3) S2 (anti-CD28) conjugated surface. Dynamic Light Scattering measurements show particles approximately 2 μm diameter conjugation efficiency assays showed similar amounts bound (0.858 μg protein/mg particles) (0.939 particles). shows 20-fold proliferation when are incubated Thus, biodegradable PLGA/PBAE recapitulate natural immune interactions anti-cancer efficacy. Currently, characterizing additional physicochemical biological properties. Our biomimetic has translational relevance may also shed light on basic biology.
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ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.221.09