Assessment of Gene Expression Profile by cDNA Microarray Analysis

Assessment of gene expression profile by cDNA microarray analysis.

With the advent of cDNA microarray technology, variations in cellular transcriptional events can now be monitored simultaneously, and expression profile at each functional state can be established and compared to one another. Here we illustrate gene expression profile analysis of a cisplatin-resistant ovarian carcinoma cell line (A2780CP)and its parental A2780 cell line, and compare them to ass...

cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle

By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of southern blotting to investigate the number of copies of these genes in the genome of swine indicated that GF I, GF III, and GF V existed as one copy and GF II, and GF IV existed as more than two copies...

cDNA microarray analysis of pigment epithelium-derived factor-regulated gene expression profile in prostate carcinoma cells.

OBJECTIVES To clarify molecular mechanisms involved in the action of pigment epithelium-derived factor (PEDF) in hormone insensitive prostate cancer cells. METHODS Total ribonucleic acid from untreated and PEDF-treated cells was subjected to microarray analysis using BioStar 8464 microarray. Real-time polymerase chain reaction analysis was conducted to confirm the microarray data. RESULTS T...

cDNA MICROARRAY TECHNOLOGY FOR THE ANALYSIS OF GENE EXPRESSION

DNA microarray technology is a new and powerful tool for studying a genome-wide gene expression in many organisms. This technology is a high throughput, highly parallel RNA expression assay technique that permits quantitative analysis of RNAs transcribed from both known and unknown genes. This review describes array manufacturing, experimental design, and its application to gene discovery and e...

cDNA Microarray Data Normalization