Assessing protein coding region integrity in cDNA sequencing projects

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessing protein coding region integrity in cDNA sequencing projects

MOTIVATION In cDNA sequencing projects, it is vital to know whether the protein coding region of a sequence is complete, or whether errors have occurred during library construction. Here we present a linear discriminant approach that predicts this completeness by estimating the probability of each ATG being the initiation codon. RESULTS Because of the current shortage of full-length cDNA data...

متن کامل

Quantifying unobserved protein-coding variants in human populations provides a roadmap for large-scale sequencing projects

As new proposals aim to sequence ever larger collection of humans, it is critical to have a quantitative framework to evaluate the statistical power of these projects. We developed a new algorithm, UnseenEst, and applied it to the exomes of 60,706 individuals to estimate the frequency distribution of all protein-coding variants, including rare variants that have not been observed yet in the cur...

متن کامل

Cloning and sequencing of chickpea cDNA coding for threonine deaminase.

TD (EC 4.2.1.16) is the enzyme catalyzing the conversion of L-Thr to a-ketobutyrate, the first step of the pathway leading to the biosynthesis of the essential amino acid, L-Ile. Regulation of TD activity by Ile was the first recognized instance of allosteric feedback regulation by the end product of a biosynthetic pathway (Umbarger, 1956). The substrate L-Thr is a feedback inhibitor of asparto...

متن کامل

Cloning and sequencing of liver cDNA coding for bovine protein C.

cDNA coding for protein C has been cloned from a bovine liver library in plasmid vector pBR322 and its sequence has been determined. Two overlapping clones code for the entire light and heavy chains of the mature protein, as well as a previously unreported connecting dipeptide (Lys-Arg) and a 39-amino acid leader peptide region. Identification and characterization of the clones establishes the ...

متن کامل

Quantifying the unobserved protein-coding variants in human populations provides a roadmap for large-scale sequencing projects

James Zou, Gregory Valiant, Paul Valiant, Konrad Karczewski, Siu On Chan, Kaitlin Samocha, Monkol Lek, Exome Aggregation Consortium, Shamil Sunyaev, Mark Daly, Daniel G MacArthur Microsoft Research, One Memorial Drive, Cambridge MA, USA Computer Science Department, Stanford University, Palo Alto CA, USA Computer Science Department, Brown University, Providence RI, USA Analytic and Translational...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Bioinformatics

سال: 1998

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/14.5.384