Aptamer-based surface enhanced Raman spectroscopy (SERS) for the rapid detection of Salmonella Enteritidis contaminated in ground beef
نویسندگان
چکیده
A rapid and specific aptamer-based surface enhanced Raman spectroscopy (SERS) for the detection of S. enterica at a serovar level was developed. The aptamer modified in this research with an additional short chain adenine fluorescein molecule. SERS method could differentiate between subsp. Enteritidis (S. Enteritidis) Gaminara Gaminara) allowed in-vitro 3 log CFU/mL. characteristic peaks 1184 730 cm−1 were used as reporters presence it binds specifically to that bacterium. Whereas, those not observed spectra Gaminara, suggesting but Gaminara. efficacy detect contaminated ground beef (93% lean) also determined. increase peak intensity 726-732 (4 CFU/g) inoculated samples apparently confirmed can be promising tool total running time 4 h.
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ژورنال
عنوان ژورنال: Lebensmittel-Wissenschaft & Technologie
سال: 2021
ISSN: ['1096-1127', '0023-6438']
DOI: https://doi.org/10.1016/j.lwt.2021.111937