Anti-Metastatic Activity of Tagitinin C from Tithonia diversifolia in a Xenograft Mouse Model of Hepatocellular Carcinoma

Sesquiterpenoid tagitinin C, present in Tithonia diversifolia leaves, has been known to have anti-hepatoma properties. Therefore, we investigated the anti-metastatic potential of C xenograft models hepatocellular carcinoma (HCC). We isolated from a methanolic extract leaves T. diversifolia. HepG-2 and Huh 7 hepatoma cells were treated with cell viability, migration, matrix metalloproteinase (MPP) activity assessed using 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide assay, scratch migration MMP respectively. used magnetic resonance spectroscopy determine tumorigenicity xenografts inoculated Hep-G2 cells. Tagitinin was cytotoxic against cells, IC50 values 2.0 ± 0.1 µg/mL 1.2 µg/mL, respectively, it showed an effect vitro. Additionally, MRS assays revealed that (15 g/mouse/day) reduced xenografts. demonstrated significant antitumor two human lines. might be as alternative or auxiliary therapy for treatment HCC, its should further clinical settings.