Analysis of the cell cycle by flow cytometry
نویسندگان
چکیده
منابع مشابه
Analysis of cell cycle by flow cytometry.
Described are four widely used procedures to analyze the cell cycle by flow cytometry. The first two are based on univariate analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4',6'-diamidino-2-phenylindole (DAPI) and deconvolution of the cellular DNA content frequency histograms. This approach reveals distribution of cells in three major phases of the...
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The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells i...
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Measurement of cellular DNA content and the analysis of the cell cycle can be performed by flow cytometry. Protocols for DNA measurement have been developed including Bivariate cytokeratin/DNA analysis, Bivariate BrdU/DNA analysis, and multiparameter flow cytometry measurement of cellular DNA content. This review summarises the methods for measurement of cellular DNA and analysis of the cell cy...
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The main approach used by large pharmaceutical companies for the design of a new drug is based on identifying an initial specific biological target that has been adequately validated. Typically biochemical or cellular assays are used by applying high-throughput screening methods (HTS) to identify hits (HitID) from large chemical libraries in the case of new chemical entities (NCEs) in order to ...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2007
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.41