Alleviation of C⋅C Mismatches in DNA by the Escherichia coli Fpg Protein
نویسندگان
چکیده
منابع مشابه
Kinetics of excision of purine lesions from DNA by Escherichia coli Fpg protein.
The kinetics of excision of damaged purine bases from oxidatively damaged DNA by Escherichia coli Fpg protein were investigated. DNA substrates, prepared by treatment with H2O2/Fe(III)-EDTA or by gamma-irradiation under N2O or air, were incubated with Fpg protein, followed by precipitation of DNA. Precipitated DNA and supernatant fractions were analyzed by gas chromatography/isotope-dilution ma...
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The Escherichia coli Fpg protein is involved in the repair of oxidized residues. We examined, by targeted mutagenesis, the effect of the conserved lysine residue at position 57 upon the various catalytic activities of the Fpg protein. Mutant Fpg protein with Lys-57-->Gly (K57G) had dramatically reduced DNA glycosylase activity for the excision of 7,8-dihydro-8-oxo-guanine (8-oxoG). While wild t...
متن کاملLocation of the fpg gene on the Escherichia coli chromosome.
The fpg gene of E. coli specifies a DNA glycosylase that excises a ring-opened form of N7-methylguanine (2-6-diamino-4hydroxy-5-N-methylformamidopyrimidine [Fapy]) from the chromosome. This gene has been isolated, on plasmids that overproduce the Fapy-DNA glycosylase activity, and sequenced; the protein has been purified to physical homogeneity (1, 2). However, no fpg mutants are known and the ...
متن کاملComparison of the levels of 8-hydroxyguanine in DNA as measured by gas chromatography mass spectrometry following hydrolysis of DNA by Escherichia coli Fpg protein or formic acid.
8-hydroxyguanine (8-OH-Gua) is one of many lesions generated in DNA by oxidative processes including free radicals. It is the most extensively investigated lesion, due to its miscoding properties and its potential role in mutagenesis, carcinogenesis and aging, and also to the existence of analytical methods using HPLC and gas chromatography mass spectrometry (GC/MS). Some studies raised the pos...
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this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2021
ISSN: 1664-302X
DOI: 10.3389/fmicb.2021.608839