A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes
نویسندگان
چکیده
منابع مشابه
Diagnostic application of padlock probes—multiplex detection of plant pathogens using universal microarrays
Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP circularization by ligation. PLPs provide extremely specific target recognition, which is followed by universal amplification and microarray detection. Since target recognition is separated from downstre...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2012
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-11-11-0309