A SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CHOLINE DEHYDROGENASE

A spectrophotometric method for the determination of choline dehydrogenase.

The increased oxygen uptake of liver suspensions on the addition of acetylcholine, which was reported by Bernheim and Bernheim in 1933 (2), was shown by Mann and Quastel (3) to be due to the oxidation of choline. Betaine aldehyde was identified as the oxidation product (3). The choline oxidase (CO) system was found by Marm, Woodward, and Quastel (4) to consist partly of a typical dehydrogenase....

AMPEROMETRIC MICROBIOSENSOR FOR THE DETERMINATION OF CHOLINE

The construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. The procedure of choline determination involved the electrochemical detection of enzymatically generated hydrogen peroxide. This microsensor has a linear response between 0.038-0.1 mM choline chloride. The...

A simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde

A Highly Sensitive Kinetic Spectrophotometric Method for the Determination of Ascorbic Acid in Pharmaceutical Samples

In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...

A Highly Sensitive Kinetic Spectrophotometric Method for the Determination of Ascorbic Acid in Pharmaceutical Samples

In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...