A preliminary studio for the F gen of Canine Distemper Virus as target for phylogenetic analysis

The Canine Distemper is one of the main infectious diseases in domestic dogs. introduction live attenuated-viral vaccines has helped to maintain disease under control. However, past few decades it been observed worldwide a rising number cases even vaccinated animals. Virus lineages circulating world have described based on hemagglutinin analyses, due its high degree genetic variability. Recently, new studies reported greater variations amino acidic sequence region fusion protein. In order determine variability field strains comparison with and from other lines, this dissertation genomic Fsp canine distemper virus protein gene analyzed. With purpose, Polymerase Chain Reaction reverse transcription, capable amplifying variable region, was implemented, identified through nucleotide sequence. These sequences were compared vaccine known lineages. Additionally, phylogenetic tree built for region. results nucleotides show that more homology strain Onderstepoort according phylogeny, would belong America 1 lineage.