A common reference for cDNA microarray hybridizations

A common reference for cDNA microarray hybridizations.

Comparisons of expression levels across different cDNA microarray experiments are easier when a common reference is co-hybridized to every microarray. Often this reference consists of one experimental control sample, a pool of cell lines or a mix of all samples to be analyzed. We have developed an alternative common reference consisting of a mix of the products that are spotted on the array. Po...

cDNA Microarray Data Normalization

Importance of replication in microarray gene expression studies: statistical methods and evidence from repetitive cDNA hybridizations.

We present statistical methods for analyzing replicated cDNA microarray expression data and report the results of a controlled experiment. The study was conducted to investigate inherent variability in gene expression data and the extent to which replication in an experiment produces more consistent and reliable findings. We introduce a statistical model to describe the probability that mRNA is...

Standardization of cDNA microarray technology for toxicogenomics; essential data for initiating cDNA microarray studies

In recent years, the DNA microarray has become one of the most powerful tools for toxicogenomic studies. However, microarray technology is still in its early stages, and standardized analytical methods of cDNA microarray analysis have not been clearly established. These differences in methodology can result in data variability in gene expression profiling. Although many analytical methods have ...

cDNA Microarray Strategy for the Construction of the cDNA Array Preparation of Subtraction Libraries Prescreening of Library Clones cDNA Microarray Production cDNA Microarray Composition