88P The effect of flubendazole on microtubule polymerization and post-translational modifications

Glioblastoma multiforme (GBM) is one of the most aggressive primary brain tumors in adults with insufficient current treatment options and very poor prognosis. Aggressive behavior GBM cells their therapeutic resistance are based on complex biological features including changes microtubule cytoskeleton. Microtubule targeting belongs to fundamental approaches cancer treatment, previously described overexpression certain tubulin isoforms suggests possible benefit use agents, such as repurposed compound flubendazole (FLU). This benzimidazole was shown inhibit a range solid tumor leukemic effect structure polymerization. The aim this study investigate FLU polymerization two cell lines (A172 T98G) examine appearance posttranslational modifications after treatment. In FLU-exposed growing ends were detected via EB1/EB3-specific immunofluorescence staining. microtubules studied fluorescent microscopy subsequent image processing analysis (ImageJ). relative quantification performed by Western blotting. reduced abundance EB1/EB3-labeled indicating inhibition concentration-dependent manner. also caused significant organization modifications. Our results demonstrate that exerts its inhibiting polymerization, well inducing network. Targeting cytoskeleton could offer strategic advantage for improving outcome therapy should be further investigated.