γ-H2AX formation in response to interstrand crosslinks requires XPF in human cells
نویسندگان
چکیده
منابع مشابه
DNA Interstrand Crosslinks Repair in Mammalian Cells
We studied the formation of double strand breaks (DSBs) as intermediates in the repair of DNA interstrand crosslinks (ICLs) by homologous recombination (HR). The plasmid EGFP-N1 was crosslinked with trioxsalen to give one ICL per plasmid on average. HeLa cells were transfected with the crosslinked plasmids and the ICL repair was monitored by following the restoration of the GFP expression. It w...
متن کاملRPA activates the XPF‐ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks
During replication-coupled DNA interstrand crosslink (ICL) repair, the XPF-ERCC1 endonuclease is required for the incisions that release, or "unhook", ICLs, but the mechanism of ICL unhooking remains largely unknown. Incisions are triggered when the nascent leading strand of a replication fork strikes the ICL Here, we report that while purified XPF-ERCC1 incises simple ICL-containing model repl...
متن کاملMismatch repair participates in error-free processing of DNA interstrand crosslinks in human cells.
DNA interstrand crosslinks (ICLs) present formidable blocks to DNA metabolic processes and must be repaired for cell survival. ICLs are induced in DNA by intercalating compounds such as the widely used therapeutic agent psoralen. In bacteria, both nucleotide excision repair (NER) and homologous recombination are required for the repair of ICLs. The processing of ICLs in mammalian cells is not c...
متن کاملSnm1B/Apollo functions in the Fanconi anemia pathway in response to DNA interstrand crosslinks.
Fanconi anemia (FA) is an inherited chromosomal instability disorder characterized by childhood aplastic anemia, developmental abnormalities and cancer predisposition. One of the hallmark phenotypes of FA is cellular hypersensitivity to agents that induce DNA interstrand crosslinks (ICLs), such as mitomycin C (MMC). FA is caused by mutation in at least 14 genes which function in the resolution ...
متن کاملThermal stability of DNA with interstrand crosslinks.
Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long DNAs caused by ICLs taking into account crosslinking effect in itself and con...
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ژورنال
عنوان ژورنال: DNA Repair
سال: 2006
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2006.03.009